A bacterial negative transcription regulator binding on an inverted repeat in the promoter for epothilone biosynthesis

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A bacterial negative transcription regulator binding on an inverted repeat in the promoter for epothilone biosynthesis

BACKGROUND Microbial secondary metabolism is regulated by a complex and mostly-unknown network of global and pathway-specific regulators. A dozen biosynthetic gene clusters for secondary metabolites have been reported in myxobacteria, but a few regulation factors have been identified. RESULTS We identified a transcription regulator Esi for the biosynthesis of epothilones. Inactivation of esi ...

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Regulation of mga transcription in the group A streptococcus: specific binding of mga within its own promoter and evidence for a negative regulator.

Transcription of mga, encoding the multiple virulence gene regulator of the group A streptococcus, is positively autoregulated. This regulation requires a DNA region (Pmga) that contains both a promoter proximal to mga (P2) and a promoter located further upstream (P1). To determine if Mga has a direct role in this process, its ability to bind to specific sequences within Pmga was tested. A puri...

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Tetrapyrroles such as chlorophylls and bacteriochlorophylls play a fundamental role in the energy absorption and transduction activities of photosynthetic organisms. Because of these molecules, however, photosynthetic organisms are also prone to photooxidative damage. They had to evolve highly efficient strategies to control tetrapyrrole biosynthesis and to prevent the accumulation of free inte...

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Regulator trafficking on bacterial transcription units in vivo.

The trafficking patterns of the bacterial regulators of transcript elongation sigma(70), rho, NusA, and NusG on genes in vivo and the explanation for promoter-proximal peaks of RNA polymerase (RNAP) are unknown. Genome-wide, E. coli ChIP-chip revealed distinct association patterns of regulators as RNAP transcribes away from promoters (rho first, then NusA, then NusG). However, the interactions ...

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ژورنال

عنوان ژورنال: Microbial Cell Factories

سال: 2017

ISSN: 1475-2859

DOI: 10.1186/s12934-017-0706-9